Structural Determinants for Specific Recognition by T4 Endonuclease V
نویسندگان
چکیده
منابع مشابه
Structural Determinants for Specific Recognition
DNA glycosylases catalyze the scission of the N-glycosyl bond linking either a damaged or mismatched base to the DNA sugar phosphate backbone. T4 endonuclease V is a glycosylase/apurinic (AP) lyase that is specific for UV light-induced cis-syn pyrimidine dimers. As a proposed transition state analog/inhibitor for glycosylases, a phosphoramidite derivative containing a pyrrolidine residue has be...
متن کاملThe role of base flipping in damage recognition and catalysis by T4 endonuclease V.
The process of moving a DNA base extrahelical (base flipping) has been shown in the co-crystal structure of a UV-induced pyrimidine dimer-specific glycosylase, T4 endonuclease V, with its substrate DNA. Compared with other enzymes known to use base flipping, endonuclease V is unique in that it moves the base opposite the target site extrahelical, rather than moving the target base itself. Utili...
متن کاملAP site structural determinants for Fpg specific recognition.
The binding of Escherichia coli and Lactococcus lactis Fapy-DNA glyosylase (Fpg) proteins to DNA containing either cyclic or non-cyclic abasic (AP) site analogs was investigated by electrophoretic mobility shift assay (EMSA) and by footprinting experiments. We showed that the reduced AP site is the best substrate analog for the E.coli and L.lactis enzymes ( K Dapp = 0.26 and 0.5 nM, respectivel...
متن کاملProcessive action of T4 endonuclease V on ultraviolet-irradiated DNA.
The action of the dimer-specific endonuclease V of bacteriophage T4 was studied on UV-irradiated, covalently-closed circular DNa. Form I ColE1 DNA preparations containing average dimer frequencies ranging from 2.5 to 35 pyrimidine dimers per molecule were treated with T4 endonuclease V and analysed by agarose gel electrophoresis. At all dimer frequencies examined, the production of form III DNA...
متن کاملC-Terminal Sequence Determinants of T4 Bacteriophage Tail Fiber Adhesin for Specific Lipopolysaccharide Recognition
bacteria strains. Their ability to a specific recognition of bacteria is used in many fields of science and technology such as bacteria identification by phage typing or as bacteria detection sensors [1]. They are also good model systems to study viral infection and replication, gene transfer, proteins folding and assembly. Bacteriophage T4 was isolated on Escherichia coli B (E. coli B) by Deme...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.50.32147